DNA Polymerase e - More Than a Polymerase
نویسندگان
چکیده
منابع مشابه
Sequence similarity of bacteriophage SP02 DNA polymerase with E. coli DNA polymerase I.
All DNA polymerases known to date are classified into three families, based on evolutionary relatedness (1). The E.coli DNA polymerase I and the 77 phage DNA polymerase share sequence similarity for regions including DNA binding domain (2) and are classified as family A (1). Family B includes cellular and DNA virus polymerases of eukaryotes as well as polymerases of some bacteriophages includin...
متن کاملOpposed Steric Constraints in Human DNA Polymerase and E. coli DNA Polymerase I
DNA polymerase selectivity is crucial for the survival of any living species, yet varies significantly among different DNA polymerases. Errors within DNA polymerase-catalyzed DNA synthesis result from the insertion of noncanonical nucleotides and extension of misaligned DNA substrates. The substrate binding characteristics among DNA polymerases are believed to vary in properties such as shape a...
متن کاملCloning and Expression of Bst DNA Polymerase I Gene in E. coli BL21
خلاصه DNA پلیمرازها علاوه بر کاربردشان در همسانه سازی و تصحیح ، در انواع تکنیک های ملکولی مانند تکثیر DNA ، جهش های نقطه ای ، توالی یابی DNA ، انواع مختلف PCR ، LAMP و ...اهمیت دارند. پس از کشف PCR تلاش هایی مبنی بر تشخیص و جداسازی آنزیم های مقاوم به دماهای بالا که توانایی تکثیر موثر DNA در دماهای بالا انجام گرفت. در این پژوهش ، سویه Geobacillus stearothermophilus strain 10 به منظور...
متن کاملSPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
متن کاملDirected evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase.
The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity. The Stoffel fragment (SF) of Thermus aquaticus DNA polymerase I is displayed on a filamentous phage by fusing it to a pIII coat protein, and the substra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Scientific World JOURNAL
سال: 2003
ISSN: 1537-744X
DOI: 10.1100/tsw.2003.08